Description
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Lysosomal Associated Membrane Protein 2 Antibody (Anti-LAMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/-10nm. The concentration of Anti-Lysosomal Associated Membrane Protein 2 Antibody (Anti-LAMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve